Rapid Cloning of PCR-Derived RAPD Probes

Rapid cloning of PCR-derived RAPD probes.

The random-amplified polymorphic DNA (RAPD) technique (5,7) is one of the most useful methods for species identification and studies on the genetic structure of populations of microand macro-parasites. This method generally provides numerous markers that must be cloned and labeled to be used as probes (1,4). These probes can be used to test the specificity and the polymorphism of the RAPD marke...

Analysis of primer-derived, nonspecific amplification products in RAPD-PCR.

Recently, we applied the randomamplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (17), also known as arbitrarily primed PCR (AP-PCR) (16), procedure to fingerprint genomes of various varieties and wild relatives of sugarcane (Saccharum spp.) (4). Unlike restriction fragment length polymorphism (RFLP) (2), RAPD-PCR is a simpler, faster, less laborious and less expensive procedure. H...

Rapid and reliable cloning of PCR products.

Hetero-stagger cloning: efficient and rapid cloning of PCR products.

A variety of methods have been developed for cloning PCR products, including blunt-end cloning (1), restriction cut back (2), ligation-independent cloning (3), uracil DNA–glycosylase (UDG) treatment of uracil-containing deoxyoligonucleotide primers (4,5) and TA cloning (6–8). Blunt-end cloning of PCR products often requires treatment of PCR products to polish the ends (9). Even with treatments,...

Rapid cloning of insect transposon insertion junctions using 'universal' PCR.

Very highly degenerate primers with short specific 3' anchor sequences and 5' adaptors were used in conjunction with nested specific primers to amplify large numbers of unknown insertion junctions of the insect retrotransposon Woot, using genomic DNA as template for the polymerase chain reaction (PCR). This technique, sometimes referred to as universal PCR, is a powerful method for molecular ch...